Open Research Data Portal
DOI for Scientific and Technical Data
https://www.doi.org/10.4228/ZALF.DK.92
Title
Rotifers negative phototaxis under UVA stress
Citation
Colangeli, Pierluigi et al. (2019) Rotifers negative phototaxis under UVA stress https://www.doi.org/10.4228/ZALF.DK.92
Publisher
Leibniz Centre for Agricultural Landscape Research (ZALF)
Contributor(s)
ContactPerson: Colangeli, Pierluigi (Leibniz Centre for Agricultural Landscape Research (ZALF), Müncheberg (Germany), GRID: 433014.1
and University of Potsdam)
DataCollector: Colangeli, Pierluigi (Leibniz Centre for Agricultural Landscape Research (ZALF), Müncheberg (Germany), GRID: 433014.1)
Supervisor: Weithoff, Guntram (University of Potsdam)
Creator(s)
Colangeli, Pierluigi (Leibniz Centre for Agricultural Landscape Research (ZALF), Müncheberg (Germany), GRID: 433014.1 and University of Potsdam)
Weithoff, Guntram (University of Potsdam)
Schlaegel, Ulrike (University of Potsdam
Obertegger, Ulrike (ORCID: 0000-0002-4057-9366; Fondazione Edmund Mach, GRID: 424414.3)
Petermann, Jana (ORCID: 0000-0002-3898-5656; University of Salzburg, GRID: 7039.d)
Tiedemann, Ralph (University of Potsdam, GRID: 11348.3f)
Subject(s)
AGROVOC: Biodiversity; Evolution
GEMET: biodiversity; ecological community; landscape ecology; evolution; animal behaviour;
Description
Abstract:
Ultraviolet radiation (UVR) is an environmental stressor in several ecosystems and can affect organisms’ survival and reproduction, and community structure. Rotifers cope with UVR stress adopting preventive behavioral and metabolic mechanisms. However, the demonstration of an immediate behavioral response in rotifers is missing. We investigated the short-term response of rotifers to UVR, by combining video analysis and movement ecology methods, in three common species: Brachionus calyciflorus, Keratella cochlearis, and Keratella quadrata. We recorded the behavior of B. calyciflorus (both sexes), K. quadrata, and K. cochlearis (females) exposed to white light, and to intermittent cycles of UVR (30:30 s). Individual trajectories were extracted from videos with open-source software. We found that B. calyciflorus females exposed to UVR exhibited strong negative phototaxis with increased swimming speed, and a weak positive phototaxis in males. Keratella cochlearis and K. quadrata showed a weaker response. Our study reveals a species-specific behavioral response to UVR in rotifers. Furthermore, we highlight how sexual dimorphism in B. calyciflorus does not only occur in morphology and movement, but also in behavioral traits. Our results help to understand zooplankton community dynamics by providing a mechanistic explanation of UVR response in one major zooplankton taxonomic group.

Methods:
Morphological and movement data obtained from video recordings. The output parameters are generated and automatically labelled by ImageJ. Please, refer to the following page for further details: https://imagej.nih.gov/ij/docs/guide/146-30.html#sub:Set-Measurements...
Funding Reference
funderName: German Research Foundation, GRID: 424150.6
awardNumber: DFG GRK 2118/1
awardTitle: BioMove Research Training Group
Language
English
Related Identifier
isDocumentedBy: DOI https://www.doi.org/10.1007/s10750-018-3801-y
Alternate Identifier
PB08
Geolocation
Quillow, Uckermark, Germany
Data Format
txt
Type of Resource
Dataset: table
Rights
Creative Commons Attribution 4.0
Structure of Data Table(s)

Tables BCA, KCO, KQU

Column name Parameter Unit Description Instrument Method Reference

file

-- original video file - - -

X

particle position pixel position of the particles across the sensor Java image processing https://imagej.nih.gov/ij/docs/guide/146-30.html

Y

particle position pixel position of the particles across the sensor Java image processing https://imagej.nih.gov/ij/docs/guide/146-30.html

frame

- - number of frame Java image processing https://imagej.nih.gov/ij/docs/guide/146-30.html

obs

- - number of observations - - -

Area

area µm² area of the particle Java image processing https://imagej.nih.gov/ij/docs/guide/146-30.html

Mean

grey value RGB mean of detection signal and grey value Java image processing https://imagej.nih.gov/ij/docs/guide/146-30.html

Min

grey value RGB minimum of detection signal and grey value Java image processing https://imagej.nih.gov/ij/docs/guide/146-30.html

Max

grey value RGB maximum of detection signal and grey value Java image processing https://imagej.nih.gov/ij/docs/guide/146-30.html

Perimeter

perimeter µm perimeter of the particle Java image processing https://imagej.nih.gov/ij/docs/guide/146-30.html

Major

  µm major axis Java image processing https://imagej.nih.gov/ij/docs/guide/146-30.html
Minor   µm minor axis Java image processing https://imagej.nih.gov/ij/docs/guide/146-30.html
Angle   - morphological index Java image processing https://imagej.nih.gov/ij/docs/guide/146-30.html
Circ   - morphological index Java image processing https://imagej.nih.gov/ij/docs/guide/146-30.html
AR   - morphological index Java image processing https://imagej.nih.gov/ij/docs/guide/146-30.html
Round   - morphological index Java image processing https://imagej.nih.gov/ij/docs/guide/146-30.html
Solidity   - morphological index Java image processing https://imagej.nih.gov/ij/docs/guide/146-30.html
id   - morphological index R statistical computing http://bemovi.info/; https://www.r-project.org/
trajectory   - morphological index R statistical computing http://bemovi.info/; https://www.r-project.org/
step_length distance µm travel distance between consecutive frames R statistical computing http://bemovi.info/; https://www.r-project.org/
step_duration duration sec duration of a step R statistical computing http://bemovi.info/; https://www.r-project.org/
step_speed speed µm/s speed of a step R statistical computing http://bemovi.info/; https://www.r-project.org/
gross_disp sum of distances µm sum of all distances R statistical computing http://bemovi.info/; https://www.r-project.org/
abs_angle angle rad absolute angle of the trajectory R statistical computing http://bemovi.info/; https://www.r-project.org/
rel_angle angle rad relative angle of the trajecotry,
-999 means not available
R statistical computing http://bemovi.info/; https://www.r-project.org/
clone - - in this case represents a species R statistical computing http://bemovi.info/; https://www.r-project.org/
replica - -
number of replicate
R statistical computing http://bemovi.info/; https://www.r-project.org/
net_disp displacement µm net displacement R statistical computing http://bemovi.info/; https://www.r-project.org/

View Sample Data
Table BCA
Table KCO
Table KQU
Download Link
Download dataset as zip file
Citation
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This data by ZALF is licensed under the Creative Commons Attribution 4.0Creative Commons License